Synergistic Effect of 6-Mercaptopurine and 6-Methylmercaptopurine Ribonucleoside on the Levels of Adenine Nucleotides of Sarcoma 180 Cells1
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چکیده
The synergistic antitumor action of 6-mercaptopurine (6-MP) and 6-methylmercaptopurine ribonucleoside (MMPR) is confirmed with Sarcoma 180 ascites cells. Striking decreases are seen in the adenine nucleotide concentrations in cells incubated for 1 hr with this drug combination, and lesser effects are seen on the guanine nucleotide concentrations. Still greater decreases in adenine nucleotide levels are observed when the cells are preincubated for 30 min with MMPR before addition of 6-MP. The 5'-monophosphate nucleotides of 6-MP and MMPR
منابع مشابه
Synergistic effect of 6-mercaptopurine and 6-methylmercaptopurine ribonucleoside on the levels of adenine nucleotides of Sarcoma 180 cells.
The synergistic antitumor action of 6-mercaptopurine (6-MP) and 6-methylmercaptopurine ribonucleoside (MMPR) is confirmed with Sarcoma 180 ascites cells. Striking decreases are seen in the adenine nucleotide concentrations in cells incubated for 1 hr with this drug combination, and lesser effects are seen on the guanine nucleotide concentrations. Still greater decreases in adenine nucleotide le...
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of MMPR forms rapidly to levels of 1 to 2 mM in the tumor cells, and the steady-state levels of 5-phosphoribosyl 1-pyrophosphate increase 4to 5-fold in 6 to 12 hr. This permits a significantly greater synthesis of 6-thioguanosine monophosphate (6-thioGMP) after injection of 6-thioguanine. These findings are similar to those in previous reports concerning the synergism between 6-mercaptopurine a...
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